Gel Electrophoresis Reagents and Buffers
Sigma Aldrich Fine Chemicals Biosciences ATX Ponceau S red staining1L
ATX ATX Ponceau S red staining1L
Sigma Aldrich Fine Chemicals Biosciences PCR 100 bp Low Ladder for
Sigmas PCR 100 bp Low Marker contains 10 dsDNA fragments from 100 - 1 000 bases evenly spaced in 100 bp repeats. Two ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose gel.
Sigma Aldrich Fine Chemicals Biosciences Acrylamide/Bis-acrylamide
ACRYLAMIDEBIS Acrylamide/Bis-acrylamide
Sigma Aldrich Fine Chemicals Biosciences 1 kb DNA Ladder for DNA el
Sigmas 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb. In nucleic acid gel electrophoresis five mul of the marker should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting. All DNA markers can be stained with ethidium bromide SYBR(R) Green and Nancy-520.
Sigma Aldrich Fine Chemicals Biosciences Phosphate-Citrate Buffer w
PHOSPHATE Phosphate-Citrate Buffer w
Sigma Aldrich Fine Chemicals Biosciences DirectLoadTM 1 kb DNA Ladd
Sigmas DirectLoad(TM) 1 kb Ladder contains 11 fragments consisting of 500 bp repeats from 0.5 to 3 kb 1 kb repeats from 3 to 6 kb and 2 kb repeats from 6 to 10 kb. for NA electrophoresis 5 ul of the marker can be loaded directly into a single lane on an agarose or polyacrylamide gel. Suitable for use in Northern and Southern blotting.
Sigma Aldrich Fine Chemicals Biosciences GelGreenR Nucleic Acid Sta
GelGreen is a sensitive stable and environmentally safe green fluorescent nucleic acid dye specifically designed for gel staining. GelGreen has UV absorption between 250 nm and 300 nm and a strong absorption peak centered around 500 nm (figure 1). Thus GelGreen is compatible with either a 254 nm UV transilluminator or a gel reader equipped with visible light excitation (such as a 488 nm laser-based gel scanner or a Dark Reader). GelGreen is far more sensitive than SYBR Safe (figure 2). Unlike SYBR dyes which are known to be unstable GelGreen is very stable both hydrolytically and thermally.The dye is noncytotoxic and non-mutagenic at concentrations well above the working concentrations used in gel staining. GelGreen successfully passed environmental safety tests in compliance with CCR Title 22 Hazardous Waste Characterization under which GelGreen is not classified as hazardous waste.Spectral PropertiesAbsorbance Standard Transilluminator (254 nm) such as a Dark ReaderEmission Lon
